Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 1. Time schedules for establishing the three groups (9 rats in each group). Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 3. Typical microscopic pathological images of HE and Masson staining in the lung tissues of rats in each group (n = 9). (a) HE staining (×100). In the control group, pulmonary alveoli, bronchioles and blood vessels were normal, and no changes in inflammation or fibrosis were observed. In the M group, the infiltration of interstitial inflammatory cells in the lung centered on the airway was remarkable, with proliferated fibrous connective tissue and noticeable proliferated fibrous tissue and smooth muscle tissue around the bronchioles and glands. The alveolar septum was widened with occasional multinucleated giant cells (indicated by a black arrow), and loose granuloma was formed. The inflammatory cell infiltration and fibrous connective tissue proliferation along the bronchus in the M+DAPT group were less severe than those in the M group. (b) Comparison of the collagen volume fractions (CVFs) was analyzed at a magnification of 40x using ImageJ software. CVF is the percentage of the blue area of collagen occupied in the total tissue area, and a higher CVF value indicates more severe fibrosis. (c) Masson staining (×100). Normal thin layers of connective tissue were observed under the submucosa of the bronchioles in the Ctrl group. In the M group, fibrosis of the bronchus and surrounding interstitial tissue was noticeable, and fibrosis developed around the granuloma in some areas (indicated by a yellow arrow). Fibrosis in the M+DAPT group was less severe than that in the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Staining, Control, Comparison, Software

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 4. Western blotting analysis of the ligands and receptors in the Notch pathway in the three groups (9 rats in each group). (a): Analysis of key Notch pathway protein levels. (b)-(g): Levels of the receptor and ligand proteins of the Notch signaling pathway. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Western Blot, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 5. The expression of the M1 macrophage marker genes TNF-α and iNOS and the M2 macrophage marker genes Arg-1 and Mrc2 in the three groups (9 rats in each group) according to PCR. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Expressing, Marker, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 6. Th1/Th2 ratio analysis of lung tissue in the three groups (9 rats in each) according to flow cytometry. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Cytometry, Control

Journal: Mediators of Inflammation

Article Title: Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice

doi: 10.1155/2023/1195149

Figure Lengend Snippet: Flow cytometric analyses of Th17 cells in SMCs and TMCs. (a) Representative flow cytometry results of Th17 cells in SMCs in the three groups. (b) Representative flow cytometry results of Th17 cells in SMCs from EAT-A mice treated with different concentrations of DAPT in vitro. (c) Representative flow cytometry analyses of Th17 cells in TMCs from EAT-A mouse thyroids. (d) Summary of Th17 cell proportions. There were significant differences of Th17 cells in SMCs among the three groups (Welch F = 140.301, ∗ P < 0.001, Welch's ANOVA). Additionally, Th17 cell percentages in EAT-A mice were significantly higher compared to those in NC mice (31.16 ± 4.79% vs. 3.99 ± 1.59%, P < 0.001, Tamhane's T2 test). In EAT-B mice, DAPT treatment via intraperitoneal injection significantly decreased the percentage of Th17 cells (7.76 ± 1.58%) compared to EAT-A mice ( P < 0.001, Tamhane's T2 test). (e). Summary of Th17 cell percentages in four groups with different DAPT concentrations in vitro. Analyses revealed that Th17 cell percentages decreased in a concentration- dependent manner ( F = 144.293, ∗ P < 0.001, one-way ANOVA).

Article Snippet: Mice in the EAT-B group were given an intraperitoneal injection of DAPT (10 mg/kg, Sigma) 30 min before the subcutaneous injection of the immunization preparation.

Techniques: Flow Cytometry, In Vitro, Injection, Concentration Assay

Journal: Mediators of Inflammation

Article Title: Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice

doi: 10.1155/2023/1195149

Figure Lengend Snippet: IL-17A concentrations in SMC culture supernatants in vivo and in vitro. (a) Altered levels of IL-17A in SMC culture supernatants. There were significant differences among the three groups ( F = 38.882, ∗ P < 0.001, one-way ANOVA). EAT-B mice had lower IL-17A concentrations than EAT-A mice (51.62 ± 2.13 pg/ml vs. 56.02 ± 3.00 pg/ml, P < 0.001), and both EAT-A and EAT-B mice had higher IL-17A concentrations than NC mice (46.32 ± 2.16 pg/ml, both P < 0.001). (b) Dose-dependent alterations of IL-17A levels in SMC culture supernatants after treatment with different concentrations of DAPT ( F = 86.750, ∗ P < 0.001, one-way ANOVA).

Article Snippet: Mice in the EAT-B group were given an intraperitoneal injection of DAPT (10 mg/kg, Sigma) 30 min before the subcutaneous injection of the immunization preparation.

Techniques: In Vivo, In Vitro